HANDBOOK OF ELECTROPHYSIOLOGY, THE

This book serves as an extensive resource on electrophysiology, offering a detailed exploration of techniques used to study ion channels, synaptic function, and neural circuits. It provides in-depth descriptions of various patch-clamp recording methods, including single-channel, whole-cell, perforated patch, and in vivo approaches. In addition to patch-clamp techniques, the book covers intracellular sharp electrode recordings, extracellular electrophysiology, and high-density multi-electrode arrays, ensuring broad applicability across diverse experimental settings.

A key strength of this book is its focus on diverse cell types and models, including neurons, glial cells, immune cells, organoids, human iPSC-derived neurons, and intracellular organelles. It also explores advanced techniques such as robotic automation, two-photon targeted patch-clamp, and optogenetics-based circuit mapping, ensuring its relevance to contemporary neuroscience research. Designed for both novice and experienced researchers, the book provides guidance on fundamental equipment, experimental design, and troubleshooting. It serves as an essential resource for neuroscientists, electrophysiologists, and biomedical researchers investigating electrical activity at cellular and network levels.

Contents:

• Introduction:
  • How to Patch a Cell (Hovy Ho-Wai Wong, Olivier Camiré & P Jesper Sjöström)
  • Current Clamp, Voltage Clamp and Dynamic Clamp (Jean-Marc Goaillard, Ede Rancz & Nicolas Wanaverbecq)
• Patch-Clamp Recordings from Channels and Cultures:
  • Single-Channel Recordings from Planar Lipid Bilayers (Nils Drexler & Indra Schroeder)
  • Single-Channel Recordings (Jelena Baranovic & Andrew Plested)
  • Whole-Cell Patch-Clamp Recordings in Cultured Neuronal Cells (Paul Kammermeier)
  • Whole-Cell Patch-Clamp Recordings in Organotypic Slices (Mathieu Letellier & Lorenzo A Cingolani)
  • Whole-Cell Patch-Clamp Recordings in 3D Organotypic Spinal Cultures (Rossana Rauti and Laura Ballerini)
• Whole-Cell Patch-Clamp Recordings from Acute Slices:
  • Quantifying Synaptic Properties (Nicole F Wong and Matthew A Xu-Friedman)
  • Channelrhodopsin-2-Assisted Circuit Mapping and Its Applications (Leopoldo Petreanu)
  • Multiple Whole-Cell Recordings (Hovy Ho-Wai Wong, Aurore Thomazeau & P Jesper Sjöström)
  • Perforated Patch-Clamp Recordings (William Kennedy & Linda Overstreet-Wadiche)
• Whole-Cell Patch-Clamp Recordings from Sub-Cellular Compartments:
  • Presynaptic Patch Clamp Recording at the Calyx of Held Nerve Terminal (Kenneth G Paradiso & Zhiping P Pang)
  • Patching Dendrites (Alon Korngreen, Thomas Nevian & Jackie Schiller)
  • Patching Endosomes and Lysosomes (Lili Qu & Chunlei Cang)
• Whole-Cell Patch-Clamp Recordings from Non-Neuronal and Genetically Reprogrammed Cells:
  • Whole-Cell Patch Clamp Recordings in Oligodendrocyte Lineage Cells (Helena Pivoňková & Ragnhildur Thóra Káradóttir)
  • Whole-Cell Patch Clamp Recordings from Astrocytes (Annalisa Scimemi)
  • Patch-Clamp Recordings in Immune Cells (Yuqing Wang & Christine Beeton)
  • Patch-Clamp Recordings from Human Induced Pluripotent Stem Cell-Derived Neurons (Michael Zabolocki, Gabriel Kolopelnyk & Cedric Bardy)
  • Whole-Cell Patch Clamp Recordings from Organoids (Jessica Sevetson, Mor Alkaslasi & Sofie Salama)
• In Vivo Patch-Clamp Recordings:
  • Intracellular Sharp Electrode Recordings in Vertebrates (Daniel F English & Earl T Gilbert)
  • Flexible Nanopipettes for Minimally Invasive Intracellular Electrophysiology (Krishna Jayant & Rafael Yuste)
  • Blind Patch-Clamp Recordings in Rodents (Paul Chadderton, Susu Chen & Ian Duguid)
  • Head-Anchored Patch-Clamp Recordings in Freely Moving Animals (Albert K Lee, Jérôme Epsztein, Doyun Lee & Michael Brecht)
  • Two-Photon Targeted Patch and Shadow Patch Recordings in Vivo (Kazuo Kitamura & Satoshi Manita)
  • Combining Patch-Clamp Recordings with Two-Photon Imaging in Vivo (Lucy M Palmer)
  • Robotic Automation of 'Blind' and Two-Photon Targeted Patch-Clamp Electrophysiology in Vivo (Gema I Vera Gonzalez, Phatsimo O Kgwarae, Luca A Annecchino, & Simon R Schultz)
  • Single-Cell Transfection and Functional Circuit Mapping Using Patch Recordings (E Rancz)
• Extracellular Recordings:
  • Basic Electrophysiological Equipment (Headley D B & Likhtik E)
  • In Vivo Extracellular Electrophysiology (Jeremiah Y Cohen)
  • In Vivo Juxtacellular Recording and Labeling of Single Neurons (Ioannis S Zouridis, Lingjun Ding, Giuseppe Balsamo, Patricia Preston-Ferrer & Andrea Burgalossi)
  • Extracellular Recording and Stimulation of the Ventral Roots and Muscle Nerves in Isolated Spinal Cord Preparations (Melanie Falgairolle & Michael J O'Donovan)
  • In Vivo Recordings of Electroencephalograms and Local Field Potentials (Melissa CM Fasol, Katerina Hristova & Alfredo Gonzalez-Sulser)
  • High Density Multi-Electrode Array Electrophysiology in Awake Animals (Woodrow Shew, Jingwen Li & Shree Hari Gautam)

Readership: Researchers and professionals in neuroscience, electrophysiology, and biomedical sciences.